Sirna Library Screening

Adapted from hu et al.

Sirna library screening.

Genome wide sirna and microrna libraries. Skip to primary content. Freedom with custom library design. In the pursuit of gene functionality rnai library screening has become a crucial research tool used to study gene function and comprehensive signaling pathways.

It enables high throughput low cost ultra fast gene function screening disease mechanism research target discovery drug development etc. Screening often lies at the beginning of most discovery research. Cancer research 2012 14 r22. 1 transfection of cells with sirna pools is performed using reverse transfection.

The number of patients with cell types selleck screening library determined according to world health organization criteria were 2 5 7 37 and 7 for types a ab b1 b2. Sirna library screening protocol. Sirna library minimize time cost of screening using highly functional sirna. The network of possible molecular interactions leading to the causal phenotype within a given system pathway or disease is astounding.

To better understand this complexity researchers are moving toward large screening projects to identify candidate genes involved in their biological question of interest. Sirna libraries composed of large numbers of sirnas have been developed and are used to identify sets of genes functionally involved in a particular biological process or disease. Prepare sirna liposome complexes in the wells of 96 well plates. Fast and easy online configuration and ordering of plated sirna microrna reagents.

Silencer sirna is a great choice for economical screening of pre defined gene families and popular pathways like kinases cell surface receptors and dna damage response. Rna interference rnai is a conserved biological response to the presence of double stranded rna which mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids and regulates the expression of protein coding genes. A breast cancer cell line was transfected with a sirna library that targeted a variety of specific kinases. Learn more and view all available silencer sirna libraries.

Take advantage of predefined gene families including druggable genes or whole genome. Rnai screening has allowed researchers to study the functionality of genes in a cell both individually and in. Library screening using genome wide sirnas as well as micrornas allows a researcher to analyze an entire signaling pathway of one or tens of thousands of genes.